Category: Biology

Genomic DNA post Lab analysis : goal is to isolate pure genomic dna from e.coli to be used next time to amplify the phoA gene using PCR

Use CER framework for the analysis and make sure to answer the following questions:

1.Correctly classified alkaline phosphatase and described the reaction it catalyzes.

2.Accurately describes the overall goal of the project and how isolating genomic DNA fits into achieving the goal.

3.The relationship between absorbance and concentration was correctly described. Correctly made conclusions on whether their A260 readings followed this relationship.

Explained whether they had to omit any A260 readings and why.

4.Correctly assessed the accuracy of the average concentration of isolated DNA using their data and answer to question 3.

5.The purity assessment of the isolated DNA was correct, the contaminate was correctly identified, and the student used their data to justify their conclusion.

6.Correctly identified one step whose failure could lead to the contamination identified in question 5 or if the DNA was pure correctly described a step that could fail and lead to an identified type of contamination.

7.Correctly explains how RNA and protein contamination affect the calculated concentration of DNA. Explains if the calculated concentration is unaffected, increased or decreased and how contamination causes that change or no change.

8.Correctly made a final assessment of the accuracy of the average concentration of isolated DNA using all data. Identified how an inaccurate concentration and contamination will affect the next step in the project.

9.Table is included. This includes a descriptive title. All data presented in the table is calculated correctly. All calculations are included in the appendix.

Additional info:

Bacteria used: E.coli strain HB101

A: Isolation of genomic dna

Reagents : saline-edta, lysozyme, RNAseA, 5M sodium perchlorate, 20% SDS, Chloroform-isoamyl alcohol (24:1), 100% EtOH, DI water.

B. Quantification of nucleic acids : I prepared 3 dilutions of the isolated dna sample as shown below;

Sample ,dH2O, DNa sample, Final volume, Dilution factor

blank ,1000 l , 0l, 1000l , N/A

A , 900 l, 100l , 1000l, 10

B, 980 l, 20l , 1000l , 50

C , 990 l , 10l , 1000l , 100

C. Collected data: We used genesys spec to take absorbance readings at 260nm and 280nm for each dilution sample:

sample, Dilution factor, A260, A280, Concentration of dna in the cuvette , Concentration of isolated DNA, A260/A280 ratio

A, 10, 1.497, 0.781, A260X50(dsDNA), Dna in the curvette X Dilution factor, 1.917

B, 50, 0.235, 0.113 , “, ” “, 2.08

C , 100, 0.116, 0.051, ,” “, 2.27

Average DNA concentration and average a260/a280 ratio: ?

Additional note: we were aiming for a pure isolated genomic dna – 1.8, my A260/A280 ratio is too high suggesting that mine is impure and contaminated with RNA pls note the reason where it could have gone wrong

Sample dataset to be completed

a general summary of the topic 2 pts why you think it is interesting/important to study 1 pt one question that you would like your research to answer 5 pts one primary source (journal article) reference, summarizing the hypothesis, methods, and results How insect feeding behaviour changes when predators are present

a general summary of the topic 2 pts why you think it is interesting/important to study 1 pt one question that you would like your research to answer 5 pts one primary source (journal article) reference, summarizing the hypothesis, methods, and results How insect feeding behaviour changes when predators are present how Insects adapt to climate change

Draft a one-page informal business proposal in which you first define the venture for which you are seeking investment to complete a proof-of-concept test. You are seeking an investment of $150,000 from a venture capitalist, and you plan to make a diagnostic assay test based on antigen-antibody principles to accomplish a medical diagnostic of your choice. You can choose a medically relevant topic of interest, such as a test for COVID-19 aimed at the detection of particular proteins, as long as the venture seeks to detect the presence or absence of infection in the body.

Your business proposal should include a high-level explanation of how this concept could work. Develop a schematic using inputs and outputs to show which indicators the assay is reading, and how it uses those indicators to decide the state of the readout being examined. You can use diagrams, figures, flowcharts, and whatever else comes to mind to describe your idea. You will include a breakdown of the budget, which may include several parameters such as product /development, research and development, and manufacturing costs. (Obviously, we’re not working with a lot of information as to how much things will actually cost, so don’t worry too much about being completely accurate with this budget; it is more of an intellectual exercise.)