1.Whatistheroleoftheimmunesystem,andhowcanitprotecthumansfromdisease?
2.Why is the rapid detection of a disease important?
3.The ELISA links an enzyme (horseradish peroxidase) to the secondary antibody. What is the purpose of the enzyme
4.Why is it necessary to include positive and negative control samples in the assay in addition to the experimental samples?
5.Why is it necessary to wash the wells after each step?
6.When the primary antibody was added to the wells, what happened if the sample contained the antigen? What happened if it did not contain the antigen?
7.Whensecondaryantibodywasaddedtothewells,whathappenedifthesamplecontainedtheantigen? What happened if it did not contain theantigen?
8.If the sample gave a negative result (colorless) for the disease-causing agent, does this always mean that a person does not have the disease? What could cause a false-negative result?
9.If you tested positive for disease exposure, did you have direct contact with one of the original infected students? If not, what conclusions can you draw about the transmissibility of disease in a population?
10.Do
you
agree
or
disagree
with
the
following
statement:
“When
you
have
sex
with
someone,
you
are
having
sex
with
everyone
they
have
ever
had
sex
with?”
Explain
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